| Author | Message | ||
     Steve Topletz Rating: N/A |
Try asking Ken Cameron at NYBG. He does Orchid DNA testing. | ||
| Hideka Kobayashi Rating: N/A |
Haven't posted here for a while. I am too busy. This really isn't my specialization, but I did work on one marker system. Typically, researchers use some marker system such as SNIP, RAPD, AFLP, SSR, ISSR, etc., etc. You may want to Google if you are interested to know what these acronyms are. Each system can be substantially different, and they have their own unique problems. For example, RAPDs aren't all that replicable, and SNIP is costly, AFLP is way cumbersome. And it takes time to get some good data, which I wasn't all that good. What they do is, by using specialized primers and combinations of such primers, they amplify gene sequences present by PCR. I think they use SNIP for paternity testing now because of sensitivity. But anyways, when you find a paricular band that is only present in one of parent, and another unique band in the other parent in the DNA sample of the baby in question, you are sure the baby is the legitimate fusion products of two gamates. But if you don't see the unique paternal band, the baby isn't his. Maybe I oversimplified, but that's how people test genetic relatedness, etc. Getting back to orchids, you know how many species there are, right?  And you have some idea how much research money is spent on orchid taxonomy. I don't want you to dissapoint, but I don't think it probably is something easily done. Some people like Keith Barlett (spell) or Mark Chase may be interested. This really isn't about DNA testing, but you can search Entrez (http://www.ncbi.nlm.nih.gov/gquery/gquery.fcgi) about genetic sequences of orchids that have been sequenced. That kind of information is useful when you develop a marker system or even run a blast search. I am just busy, so I probably won't keep posting. But you know how to get a hold of me, right? The Paph in question looks like a hybrid to me, but it's just my humble opinion. Paph. coccineum may be present in US, and if so, isn't it 'illegal'? Here's some pics of Paph. coccineum: http://www.orchid.or.jp/orchid/people/tanaka/orchid/org/shinshu/enshinshu22.html Hideka | ||
| M. Kevin Johnson Rating: N/A |
OK folks. I thought this would be easier to find on teh net (and AOS info) then it is so I figure I would ask here. I have ome plant that I may need to verify what it is (photo on other posts). My question is when a plant is DNA tested what are the labs that do it because I can not find something readily that specializes in orchids. Because getting into the DNA is going to be far over my head in laymans terms what do they compare the samples too? Have they run DNA samples on most of the known species already? |
